Education
Degree |
Date |
Affiliation |
Major |
PhD |
2012 |
Technische Universitaet Dresden |
Biotechnology |
Master |
2001 |
Technische Universitaet Dresden |
Forestry |
Bachelor |
1991 |
Northwest Agriculture and Forestry University |
Forestry |
Research Experience
Date |
Affiliation |
Position |
2014 - |
Shandong University |
Professor |
2004 –2014 |
Technische Universitaet Dresden |
Research fellow |
2002 – 2004 |
Gene Bridges GmbH |
R&D assistant |
1991 – 1998 |
Northwest Agriculture and Forestry University |
Research assistant |
Research Interests
Research and development of recombineering
Site-specific recombination
Transposon mediated horizontal gene transfer
Gene targeting and BAC transgenesis
PKS/NRPS gene clusters engineering and heterologous expression
Viral genomic DNA library construction and vaccination
Research Projects
1. The International S&T Cooperation Program of China (2015DFE32850)
2. National Natural Science Foundation of China (31570094),
3. The Shandong Provincial Natural Science Foundation (ZR2018ZC2261)
4. National Key R&D Program of China (2018YFA0900403)
Representative Publications
1. Jia Yin, Wentao Zheng, Yunsheng Gao, Chanjuan Jiang, Hongbo Shi, Xiaotong Diao, Shanshan Li, Hanna Chen, Hailong Wang, Ruijuan Li, Aiying Li, Liqiu Xia, Yulong Yin, A. Francis Stewart, Youming Zhang and Jun Fu.（2019）“Single-Stranded DNA-Binding Protein and Exogenous RecBCD Inhibitors Enhance Phage-Derived Homologous Recombination in Pseudomonas”iScience. 14:1-14.
2. Chaoyi Song, Ji Luan, Qingwen Cui, Qiuyue Duan, Zhen Li, Yunsheng Gao, Ruijuan Li, Aiying Li, Yue-Mao Shen, Yue-Zhong Li, Adrian Francis Stewart, Youming Zhang, Jun Fu, and Hailong Wang（2019）“Enhanced heterologous spinosad production from a 79-kb synthetic multi-operon assembly”ACS Synthetic Biology, 8 (1):137-147
3. Gulce Itir Percin, Jiri Eitler, Andrea Kranz, Jun Fu, Jeffrey W. Pollard, Ronald Naumann & Claudia Waskow (2018) “CSF1R regulates the dendritic cell pool size in adult mice via embryo-derived tissue-resident macrophages”Nature Communications. 9(1):5279
4. Yuhan Zhang, Ruxin Liu, Kaiyue Tian, Zeng Wang, XiaYang, Dongsheng Gao, Youming Zhang, Jun Fu*, Hailong Wang*, Jun Zhao*（2018）“Fiber2 and hexon genes are closely associated with the virulence of the emerging and highly pathogenic fowl adenovirus 4”Emerging Microbes & Infections. 7(1):199. doi: 10.1038/s41426-018-0203-1.
5. Hailong Wang, Zhen Li，Ruonan Jia，Jia Yin, Aiying Li，Liqiu Xia，Yulong Yin, Rolf Müller, Jun Fu, A. Francis Stewart and Youming Zhang (2018)“ExoCET: Exonuclease in vitro assembly combined with RecET recombination for highly efficient direct DNA cloningfrom complex genomes”Nucleic Acids Research. 2018 Mar 16; 46(5):e28.
6. Oliver Baker, Sarah Tsurkan, Jun Fu, Barbara Klink, Andreas Rump, Mandy Obst, Andrea Kranz, Evelin Schröck, Konstantinos Anastassiadis and A. Francis Stewart (2017) “The contribution of homology arms to nuclease-assisted genome engineering.”Nucleic Acids Research 2017 Jun 5. doi: 10.1093/nar/gkx497
7. Wenli Zhang, Jun Fu, Jing Liu, Hailong Wang, Maren Schiwon, Sebastian Janz, Lukas Schaffarczyk, Lukas von der Goltz, Eric Ehrke-Schulz, Johannes Dörner, Manish Solanki, Philip Boehme, Thorsten Bergmann, Andre Lieber, Chris Lauber, Andreas Dahl, Andreas Petzold, Youming Zhang, A. Francis Stewart and Anja Ehrhardt (2017) “An Engineered Virus Library as a Resource for the Spectrum-wide Exploration of Virus and Vector Diversity”Cell Reports. 19(8):1698-1709.
8. Hailong Wang, Zhen Li, Ruonan Jia, Yu Hou, Jia Yin, Xiaoying Bian, Aiying Li, Rolf Müller, A Francis Stewart, Jun Fu & Youming Zhang (2016) "A concerted pipeline for direct cloning of biosynthetic gene clusters and engineering for heterologous expression"Nature Protocols 11(7): 1175-90.
9. Oliver Baker, Ashish Gupta, Mandy Obst, Youming Zhang, Konstantinos Anastassiadis, Jun Fu and A. Francis Stewart (2016) “RAC-tagging: Recombineering And Cas9-assisted targeting for protein tagging and conditional analyses” Scientific Reports 2016 May 24;6:25529. doi: 10.1038/srep25529.
10. Yin, J., Zhu, H., Xia, L., Ding, X., Hoffmann, T., Hoffmann, M., Bian, X., Müller, R., Fu, J., Stewart, A.F., Zhang, Y. (2015) “A new recombineering system for Photorhabdus and Xenorhabdus”Nucleic Acids Research2015 Mar 31; 43(6):e36.
11. Wang, H., Bian, X., Xia, L., Ding, X., Müller, R., Zhang, Y., Fu, J. and Stewart, A. F. (2014) “Improved seamless mutagenesis by recombineering using ccdB for counterselection"Nucleic Acids Research 2013 Dec 24.
12. Filipczyk, A., Gkatzis, K., Fu, J., Lickert, H., Anastassiadis, K. and Schroeder, T. (2013) " Biallelic Expression of Nanog Protein in Mouse Embryonic Stem Cells"Cell Stem Cell 13:12-13
13. Rostovskaya, M., Fu, J., Obst, M., Baer, I., Weidlich, S., Wang, H., Smith, A., Anastassiadis, K. and Stewart, A. F. (2012) “Transposon-Mediated BAC Transgenesis in Human ES Cells”Nucleic Acids Research, 2012 Jun 30. doi: 10.1093/nar/gks643
14. Fu, J., Bian, X., Hu, S., Wang, H., Huang, F., Seibert, P., Plaza, A., Xia, L., Müller, R., Stewart, A. F. and Zhang, Y. (2012) "Full-length RecE enhances linear-linear homologous recombination and facilitates direct cloning for bioprospecting.”Nature Biotechnology 30: 440-446.
15. Bird, A. W., Erler, A., Fu, J., Maresca, M., Heriche, J. K., Zhang, Y., Hyman, A. A. and Stewart, A. F. (2011) "High-efficiency counterselection recombineering for site-directed mutagenesis in bacterial artificial chromosomes."Nature Methods 9:103-109.
16. Nedelkova, M., Maresca, M., Fu, J., Rostovskaya, M., Thiede, C., Anastassiadis, K., Sarov, M. and Stewart, A. F. (2011). "Targeted isolation of genomic regions by recombineering."Nucleic Acids Research 39:e137.
17. Daniel, K., Lange, J., Hached, K., Fu, J., Anastassiadis, K., Roig, I., Cooke, H. J., Stewart, A. F., Wassmann, K., Jasin, M., Keeney, S. and Toth, A. (2011). "Meiotic homologue alignment and its quality surveillance are controlled by mouse HORMAD1."Nature Cell Biology 13:599-610.
18. Skarnes, W. C., Rosen, B., West, A. P., Koutsourakis, M., Bushell, W., Iyer, V., Cox, R., Jackson, D., Severin, J., Biggs, P., Thomas, M., Mujica, A., Harrow, J., Fu, J., Nefedov, M., de Jong, P., Stewart, A. F. and Bradley, A. (2011). "A conditional knockout resource for genome-wide analysis of mouse gene function."Nature 474: 337-42.
19. Fu, J., Teucher, M., Anastassiadis, K., Skarnes, W. and Stewart, A. F. (2010). "A Recombineering pipeline to make conditional targeting constructs."Methods in Enzymology 477C: 125-144.
20. Fu, J., Wenzel, S. C., Perlova, O., Wang, J., Gross, F., Tang, Z., Yin, Y., Stewart, A. F., Müller, R. and Zhang, Y. (2008)."Efficient transfer of two large secondary metabolite pathway gene clusters into heterologous hosts by transposition." Nucleic Acids Research 36: e113.
Patents
1. ZHANG, Youming；WANG， Hailong；FU, Jun; STEWART, Adrian, Francis;（08.2017）“New technique for genomic large fragment direct cloning andDNA multi-molecular assembly”PCT/CN2017/000483
2. STEWART, Adrian, Francis; FU, Jun; EHRHARDT, Anja; EHRKE-SCHULZ, Eric; ZHANG, Wenli “Adenoviral vectors” PCT/EP2017/058306
3. TU, Qiang; MÜLLER, Rolf; STEWART, Francis; FU, Jun; ZHANG, Youming.“High efficiency of electrocompetent cells prepared at room temperature” PCT/EP2013/071546
4. FU, Jun; ZHANG, Youming; STEWART, Francis.“Direct cloning of DNA fragment from genomic DNA without library construction and screening” PCT/IB2011/052549
5. ZHANG, Youming; FU, Jun; BIAN, Xiaoying; STEWART, Francis; ZIPS, Daniel; MÜLLER, Rolf “Heterologous expression of PKS/NRPS gene cluster and targeted anticancer drug delivery.” PCT/IB2010/055923
6. MARESCA, Marcello; ERLER, Axel, Steffen; FU, Jun; SEIBERT, Philipp, Martin; STEWART, Adrian, Francis; ZHANG, Youming “Method of nucleic acid recombination” PCT/IB2009/000488
7. ZHANG, Youming; MÜLLER, Rolf; STEWART, Francis; GROSS, Frank; WENZEL,Silke, C.; FU, Jun “Methods for heterologous expression of secondary metabolites” PCT/IB2005 /003650; EP1809749 (2007); US20090298117 (2009)
