Education

Research Experience

Research Interests

Development of genome engineering tools in microorganisms

Microbial natural product synthetic biology

Research Projects

2024.01-2026.12, Taishan Scholar Program of Shandong Province

2023.12-2028.11, National Key Research and Development Program of China

2021.01-2013.12, Shandong Provincial Natural Science Foundation of China

2020.01-2024.12, National Key Research and Development Program of China

2018.6-2021.06, The Fundamental Research Funds of Shandong University

2016.01-2018.12, National natural science foundation of China

2015.06-2017.05, China Postdoctoral Science Foundation Funded

Representative Publications

Wentao Zheng^#, Yandong Xia^#, Xue Wang, Shiqing Gao, Diao Zhou, Vinothkannan Ravichandran, Chanjuan Jiang, Qiang Tu, Yulong Yin, Youming Zhang, Jun Fu^*, Ruijuan Li^*, Jia Yin^*. Precise genome engineering in Pseudomonas using phage-encoded homologous recombination and Cascade-Cas3 system. Nat Protoc 2023, 18(9): 2642-2670.

Kai Gong, Maoqin Wang, Qiong Duan, Gang Li, Daojing Yong, Cailing Ren, Yue Li, Qijun Zhang, Tao Sun, Huanyun Zhang, Qiang Tu, Changsheng Wu, Jun Fu, Aiying Li, Chaoyi Song^*, Youming Zhang^*, Ruijuan Li^*. High-yield production of FK228 and new derivatives in a Burkholderia chassis. Metab Eng 2023, 75(2023): 131‒142.

Xiuling Wang, Ping Lin, Qiyao Shen, Xueyan Feng, Shouying Xu, Qijun Zhang, Yang Liu, Cailing Ren, Daojing Yong, Qiong Duan, Liujie Huo, Youming Zhang, Gang Li*, Jun Fu*, Ruijuan Li*. A highly efficient heterologous expression platform to facilitate the production of microbial natural products in Streptomyces. Microb Cell Fact 2025, 24(1):105.

Shiqing Gao^#, Xiuling Wang^#, Runyu Yang, Zhilong Bian, Daojing Yong, Shouying Xu, Liujie Huo, Qiang Tu, Youming Zhang, Jun Fu^*, and Ruijuan Li^*. Efficient genome engineering in Agrobacterium tumefaciens C58 using recombineering assisted by CRISPR/Cas9. J Biotechnol. 2025, 406: 99-104.

Xiuling Wang^#, Shiqing Gao^#, Jun Fu*, Ruijuan Li*. Heterologous expression of the nitrogen-fixing gene cluster from Paenibacillus polymyxa in Bacillus subtilis. Microorganisms 2025, 13(6), 1320.

Daojing Yong, Yingying Yu, Shuai Zhao, Shoudong Cui, Shuai Zhu, Zonglin Yang, Yue Li, Qijun Zhang, Shiqing Gao, Junchi Ma, Aiying Li, Jun Fu, Jinfeng Ni*, Youming Zhang* and Ruijuan Li*. Mechanisms of biocontrol of gray mold in postharvest pear fruits using the termite symbiont Streptomyces griseus H3950. Plant Physiol Bioch 2025, 224:109892.

Xiuling Wang, Zhuo Zhang, Jun Fu^*, Ruijuan Li^*. Genomics-driven discovery of plantariitin A, a novel lipopeptide in Burkholderia plantarii DSM9509. Molecules 2025, 30(4): 868.

Qingshu Liu^#, Ruijuan Li^#,*, Hongbo Shi, Runyu Yang, Qiyao Shen, Qingwen Cui, Xiuling Wang, Aiying Li, Youming Zhang^*, Jun Fu^*. A recombineering system in Bacillus subtilis based on the native phage recombinase pair YqaJ/YqaK. Engineering Microbiology 2023, 3(3): 100099.

Ruijuan Li, Aiying Li, Youming Zhang, Jun Fu^*. The emerging role of recombineering in microbiology. Engineering Microbiology 2023, 3(3): 100097.

Zhilong Bian, Shanshan Li, Runyu Yang, Jia Yin, Youming Zhang, Qiang Tu^*, Jun Fu^*, Ruijuan Li^*. Development of a new recombineering system for Agrobacterium species. Appl Environ Microbiol. 2022, 88(5): e0249921.

Daojing Yong, Yue Li, Kai Gong, Yingying Yu, Shuai Zhao, Qiong Duan, Cailing Ren, Aiying Li, Jun Fu, Jinfeng Ni^*, Youming Zhang^* and Ruijuan Li^*. Biocontrol of strawberry gray mold caused by Botrytis cinerea with the termite associated Streptomyces sp. sdu1201 and actinomycin D. Front Microbiol. 2022, 13:1051730.

Khorshed Alam, Yiming Zhao, Kai Gong, Lin Zhong, Md. Mahmudul Islam, Saiful Islam, Geng Li, Ruijuan Li^*, Youming Zhang^*, Aiying Li^*. Isolation, complete genome sequencing and in silico genome mining of Burkholderia for secondary metabolites. BMC Microbiol 2022, 22:323.

Khorshed Alam^#, Jinfang Hao^#, Lin Zhong, Guoqing Fan, Qing Ouyang, Md. Mahmudul Islam, Saiful Islam, Hongluan Sun, Youming Zhang, Ruijuan Li^*, Aiying Li^*. Complete genome sequencing and in silico genome mining reveal the promising metabolic potential in Streptomyces strain CS-7. Front Microbiol. 2022, 13:939919.

Wentao Zheng^#, Yandong Xia^#, Xue Wang, Shiqing Gao, Diao Zhou, Jun Fu^*, Ruijuan Li^*, Jia Yin^*. Cascade-Cas3 facilitates high-accuracy genome engineering in Pseudomonas using phage-encoded homologous recombination. Engineering Microbiology 2022, 2(4): 100046.

Kai Gong^#, Daojing Yong^#, Jun Fu, Aiying Li, Youming Zhang, Ruijuan Li^*. Diterpenoids from Streptomyces: Structures, Biosyntheses and Bioactivities. ChemBioChem. 2022, 23(17): e202200231.

Xiaotong Wang, Chaoyi Song, Qingsheng Qi, Youming Zhang^*, Ruijuan Li^*, Liujie Huo^*. Biochemical characterization of a polyethylene terephthalate hydrolase and design of a high-throughput screening for its directed evolution. Engineering Microbiology 2022, 2(2), 100020.

Ruijuan Li^#, Hongbo Shi^#, Xiaoyu Zhao, Xianqi Liu, Qiong Duan, Chaoyi Song, Hanna Chen, Wentao Zheng, Qiyao Shen, Maoqin Wang, Xue Wang, Kai Gong, Jia Yin, Youming Zhang^*, Aiying Li^*, Jun Fu^*. Development and application of an efficient recombineering system for Burkholderia glumae and Burkholderia plantarii. Microb Biotechnol 2021, 14(4): 1809‒1826.

Khorshed Alam, Md. Mahmudul Islam, Caiyun Li, Sharmin Sultana, Lin Zhong, Qiyao Shen, Guangle Yu, Jinfang Hao, Youming Zhang, Ruijuan Li^*, Aiying Li^*. Genome mining of Pseudomonas species: diversity and evolution of metabolic and biosynthetic potential. Molecules. 2021, 26, 7524.

Khorshed Alam, Md. Mahmudul Islam, Kai Gong, Muhammad Nazeer Abbasi, Ruijuan Li^*, Youming Zhang^*, Aiying Li^*. In silico genome mining of potential novel biosynthetic gene clusters for drug discovery from Burkholderia bacteria. Comput Biol Med. 2021, 140 (2022) 105046.

Caiyun Li, Khorshed Alam, Yiming Zhao, Jinfang Hao, Qing Yang, Youming Zhang^*, Ruijuan Li^*, Aiying Li^*. Mining and biosynthesis of bioactive lanthipeptides from microorganisms. Front Bioeng Biotech 2021, 9(639): 692466.

Ruijuan Li^#, Linda Helbig^#, Jun Fu, Xiaoying Bian, Jennifer Herrmann, Michael Baumann, A. Francis Stewart, Rolf Müller, Aiying Li^*, Daniel Zips^*, and Youming Zhang^*. Expressing cytotoxic compounds in Escherichia coli Nissle 1917 for tumor-targeting therapy. Res Microbiol 2019, 170(2): 74–79.

Ruijuan Li^#, Song Wang^#, Gang Li, Jinchuan Zhou, Jiaozhen Zhang, Youming Zhang, Guosheng Shi, and Hongxiang Lou^*. Four new kaurane diterpenoids from the Chinese liverwort Jungermannia comata Nees. Chem Biodivers 2016, 13(12): 1685–1690.

Ruijuan Li, Yu Zhao, Harukuni Tokuda, Xiaoming Yang, Yuehu Wang, Qian Shi, Susan L. Morris-Natschke, Hongxiang Lou^*, and Kuo-Hsiung Lee^*. Total synthesis of plagiochin G and its derivatives as potential cancer chemopreventive agents. Tetrahedron Lett 2014, 55(2014): 6500−6503.

Song Wang,^# Ruijuan Li,^# Rongxiu Zhu, Jinchuan Zhou, Zhaomin Lin, Jiaozhen Zhang, Jingyi Wu, Yaqi Kang, Susan L. Morris-Natschke, Kuo-Hsiung. Lee, and Hongxiang Lou^*. Notolutesins A−J, dolabrane-type diterpenoids from the Chinese liverwort Notoscyphus lutescens. J Nat Prod 2014, 77(9): 2081−2087.

Ruijuan Li, Rongxiu Zhu, Yu Zhao, Susan L. Morris-Natschke, Chin-Ho Chen, Song Wang, Jiaozhen Zhang, Jinchuan Zhou, Hongxiang Lou^*, and Kuo-Hsiung Lee^*. Two new cadinane-type sesquiterpenes from the Chinese liverwort Frullania serrata. Nat Prod Res 2014, 28: 1519−1524.

Ruijuan Li, Yi Sun, Bin Sun, Xiaoning Wang, Shanshan Liu, Jinchuan Zhou, Jianping Ye, Yu Zhao, Lei Liu, Kuo-Hsiung Lee, and Hongxiang Lou^*. Phytotoxic cis-clerodane diterpenoids from the Chinese liverwort Scapania stephanii. Phytochemistry 2014, 105(2014): 85−91.

Ruijuan Li,^# Zhaomin Lin,^# Yaqi Kang, Yanxia Guo, Xin Lv, Jinchuan Zhou, Song Wang, and Hongxiang Lou^*. Cembrane-type diterpenoids from the Chinese liverworts Chandonanthus hirtellus and C. birmensis. J Nat Prod 2014, 77(2): 339−345.

Ruijuan Li, Rongxiu Zhu, Yaoyao Li, Jinchuan Zhou, Jiaozhen Zhang, Song Wang, Jianping Ye, Yuehu Wang, Susan L. Morris-Natschke, Kuo-Hsiung Lee, and Hong-Xiang Lou^*. Secondary metabolites from the Chinese liverwort Cephaloziella kiaeri. J Nat Prod 2013, 76(9): 1700−1708.

Ruijuan Li, Dongxiao Guo, and Hongxiang Lou^*. A new guaiane-type sesquiterpene lactone from the Chinese liverwort Porella acutifolia subsp. tosana. Chin J Nat Med 2013, 11(1): 0074−0076.

Honors and awards

Patents

李瑞娟，宫恺，张友明，王茂芹，宋超逸，符军，李爱英。中国发明专利，一组FK228衍生物及其制备方法与应用。ZL2022111223050

李瑞娟，宋超逸，王茂芹，宫恺，张友明，符军，李爱英。中国发明专利，化合物Spiruchostatin E及其制备方法和应用。ZL2022111219040

李瑞娟，宫恺，王茂芹，宋超逸，张友明，符军，李爱英。中国发明专利，一株高产FK228的工程菌株及其构建与应用。ZL202210109315.4

符军，李瑞娟，边志龙，李珊珊，杨润雨，涂强，张友明。中国发明专利，农杆菌同源重组系统及其应用。ZL2021110882272

符军，李瑞娟，史洪波，赵晓雨，张友明。中国发明专利，伯克氏菌同源重组系统及其应用。ZL202011131275.0

张友明，李瑞娟，高运生，涂强，王宗杰。中国发明专利，一株高效异源表达Disorazole Z的工程菌株和构建该菌株的基因簇及其应用。ZL201711363593.8

张友明，李瑞娟，宋超逸，涂强。中国发明专利，一株异源表达组蛋白去乙酰化酶抑制剂的工程菌株及其应用。ZL201710813845.6