Education
Degree |
Date |
Affiliation |
Major |
PhD |
Sep. 2006-Dec. 2011 |
Shandong University |
Microbiology |
Bachelor |
Sep. 2002-July 2006 |
Shandong University |
Biotechnology |
Research Experience
Date |
Affiliation |
Position |
Jun. 2018-present |
State Key Laboratory of Microbial Technology, Shandong University |
Associate Professor |
Jul. 2016-May2018 |
Institute of Marine Science and Technology,Shandong University |
Associate Professor |
Feb. 2012-Dec. 2015 |
School of Chemistry and Chemical Engineering, Shandong University |
Post doctor |
Research Interests
1.Structural and functional analysesof new enzymes involved inmarine organic carbon degradation
2.Physiological and genetic analyses of marine unculturable microorganisms
Research Projects
1. Jan. 2018-Dec. 2020, The catalytic mechanism of phospholipase PlcP and the role of PlcP in marine carbon and phosphorus cycles, National Natural Science Foundation of China (91751101),principal investigator.
2. Jan. 2017-Dec. 2020, Catalytic mechanism and physiological function of a novel two-domain esterase containing an OsmC domain, National Natural Science Foundation of China (41676180),principal investigator.
3. Aug. 2013-Aug. 2015, Carbon sources and energy metabolism of theunculturableMCG crenarchaea revealed by analysis of BAC libraries,China postdoctoral science foundation(2013M53158), principal investigator.
Representative Publications
1.Yue Wang1,Ping-Yi Li1, Yi Zhang, Hai-Yan Cao, Yan-Jun Wang, Chun-Yang Li, Peng Wang, Hai-Nan Su, Yin Chen, Xiu-Lan Chen*, Yu-Zhong Zhang*.3,6-anhydro-L-galactose dehydrogenase VvAHGD is a member of a new aldehyde dehydrogenase family and catalyzes by a novel mechanism with conformational switch of two catalytic residues cysteine 282 and glutamate 248. Journal of Molecular Biology, 2020, doi: 10.1016/j.jmb.2020.02.008
2.Ping-Yi Li1, Yan-Qi Zhang1, Yi Zhang1, Wen-Xin Jiang, Yan-Jun Wang, Yi-Shuo Zhang, Zhong-Zhi Sun, Chun-Yang Li, Yu-Zhong Zhang, Mei Shi, Xiao-Yan Song,Long-Sheng Zhao*, Xiu-Lan Chen*.Study on a novel cold-active and halotolerant monoacylglycerol lipase widespread in marine bacteria reveals a new group of bacterial monoacylglycerol lipases containing unusual C(A/S)HSMG catalytic motifs.Frontiers in Microbiology, 2020, 11:9
3.Yan-Jun Wang1, Wen-Xin Jiang, Yi-Shuo Zhang, Hai-Yan Cao, Yi Zhang,Xiu-Lan Chen, Chun-Yang Li, Peng Wang, Yu-Zhong Zhang, Xiao-Yan Song*,Ping-Yi Li*. Structural insight into chitindegradation and thermostability of anovel endochitinase from theglycoside hydrolase family 18. Frontiers in Microbiology, 2019, 10:2457
4. Xiu-Lan Chen1, Fang Zhao1, Yong-Sheng Yue, Xi-Ying Zhang, Yu-Zhong Zhang,Ping-Yi Li*.A new group of modular xylanases in glycoside hydrolasefamily 8 from marine bacteria. Applied and Environmental Microbiology, 2018, 84: e01785-18
5.Ping-Yi Li1, Qiong-Qiong Yao1, Peng Wang, Yi Zhang, Yue Li, Yan-Qi Zhang, Jie Hao, Bai-Cheng Zhou, Xiu-Lan Chen*, Mei Shi, Yu-Zhong Zhang, Xi-Ying Zhang*. A novel subfamily esterase with a homoserine transacetylase-like fold but no transferase activity. Applied and Environmental Microbiology, 2017, 83：e00131~17
6.Ping-Yi Li1, Yi Zhang1, Bin-Bin Xie*, Yan-Qi Zhang, Jie Hao, Yue Wang, Peng Wang,Chun-Yang Li, Qi-Long Qin, Xi-Ying Zhang, Hai-Nan Su, Mei Shi, Yu-Zhong Zhang, Xiu-Lan Chen*. Structural andmechanisticinsights into theimprovement of thehalotolerance of amarinemicrobialesterase byincreasingintra- andinterdomainhydrophobicinteractions. Applied and Environmental Microbiology, 2017, 83: e01286~17
7. Yi Zhang1, Jie Hao, Yan-Qi Zhang, Xiu-Lan Chen, Bin-Bin Xie, Mei Shi, Bai-Cheng Zhou, Yu-Zhong Zhang,Ping-Yi Li*. Identification and characterization of a novel salt-tolerant esterase from the deep-sea sediment of the South China Sea, Frontiers in Microbiology, 2017, 8: 441
8. Fei Xu1, Fang Dong, Peng Wang, Hai-Yan Cao,Chun-Yang Li,Ping-Yi Li, Xiu-Hua Pang, Yu-Zhong Zhang, Xiu-Lan Chen*. Novel molecular insights into the catalytic mechanism of marine bacterial alginate lyase AlyGC from polysaccharide lyase family 6.Journal of Biological Chemistry, 2017, 292: 4457~4468
9.Ping-Yi Li1, Xiu-Lan Chen, Peng Ji, Chun-Yang Li, Peng Wang, Yi Zhang, Bin-Bin Xie, Qi-Long Qin, Hai-Nan Su, Bai-Cheng Zhou, Yu-Zhong Zhang, Xi-Ying Zhang*. Interdomain hydrophobic interactions modulate the thermostability of microbial esterases from the hormone-sensitive lipase family, Journal of Biological Chemistry, 2015, 290(17): 11188~11198
10.Ping-Yi Li1, Peng Ji, Chun-Yang Li, Yi Zhang, Guang-Long Wang, Xi-Ying Zhang, Bin-Bin Xie, Qi-Long Qin, Xiu-Lan Chen, Bai-Cheng Zhou, Yu-Zhong Zhang*. Structural basis for dimerization and catalysis of a novel esterase from the GTSAG motif subfamily of the bacterial hormone-sensitive lipase family, Journal of Biological Chemistry, 2014, 289(27): 19031~19041
11. Bing Guo1,Ping-Yi Li1, Yong-Sheng Yue, Hui-Lin Zhao, Sheng Dong, Xiao-Yan Song, Cai-Yun Sun, Wei-Xin Zhang, Xiu-Lan Chen, Xi-Ying Zhang*. Bai-Cheng Zhou, Yu-Zhong Zhang, Gene cloning, expression and characterization of a novel xylanase from the marine bacterium,Glaciecola mesophilaKMM241, Marine Drugs, 2013, 11(4): 1173~1187
12.Ping-Yi Li1, Bin-Bin Xie*, Xi-Ying Zhang, Qi-Long Qin, Hong-Yue Dang, Xu-Min Wang, Xiu-Lan Chen, Jun Yu, Yu-Zhong Zhang*. Genetic structure of three fosmid-fragments encoding 16S rRNA genes of the Miscellaneous Crenarchaeotic Group (MCG): implications for physiology and evolution of marine sedimentary archaea, Environmental Microbiology, 2012, 14(2): 467~479
Patents
1. 一种海洋适冷酯酶及其编码基因E40与应用，ZL201510025698.7，第二位。
2. 一种海洋酯酶及其编码基因E32与应用，ZL201510216087.0，第二位。
