Education

Research Experience

Research Interests

Microbial physiology, biochemistry and discoveryand molecularevolution of microbial glycoenzymes,mainly focusing on enzyme-catalyzed synthesis of oligosaccharides andglycosyl compounds.

Research Projects

Major State Basic Research Development Program of China (973 Program) (No. 2012CB822102, 2012. 01-2016. 08)

National Natural Science Foundation of China (No.31070064,2011.01-2013.12; No.31170054, 2012.01-2015.12; No. 31670062,2017.01-2020.12 ; No.31872626, 2019.1-2022.12)

Science and Technology Development Project of Shandong Province (No. 2014GSF121006, 2014.01-2016.12).

Representative Publications

1. Chunjuan He, Yougui Huang, Peng Liu, Jianhuan Wei, Yirui Yang, Li Xu*, Min Xiao*. Transcriptome analysis of genes and metabolic pathways associated with nicotine degradation in Aspergillus oryzae 112822. BMC genomics. 2019, 20(1): 86. DOI: 10.1186/s12864-019-5446-2

2. Longcheng Guo, Xiaodi Chen, Li Xu, Min Xiao*, Lili Lu*. Enzymatic Synthesis of 6’-Sialyllactose, a Dominant Sialylated Human Milk Oligosaccharide, by a Novel exo-α-Sialidase fromBacteroides fragilisNCTC9343. Appl Environ Microbiol. 2018, 84(13):e00071-18

3. Chunjuan He, Yirui Yang, Renfei Zhao, Jingyao Qu, Lan Jin, Lili Lu, Li Xu*, Min Xiao*. Rational designed mutagenesis of levansucrase fromBacillus licheniformis8-37-0-1 for product specificity study. Appl Microbiol Biotechnol. 2018, 102(7):3217-3228

4. Tingting Qi, Guofeng Gu, Li Xu, Min Xiao*, Lili Lu*. Efficient synthesis of tyrosol galactosides by theb-galactosidase fromEnterobacter cloacaeB5. Appl Microbiol Biotechnol. 2017, 101(12):4995-5003

5. Xiaodi Chen, Li Xu, Lan Jin, Bin Sun, Guofeng Gu, Lili Lu*, Min Xiao*.Efficient and regioselective synthesis of β-GalNAc/GlcNAc-lactose by a bifunctional transglycosylating β-N-acetylhexosaminidase fromBifidobacterium bifidum. Appl Environ Microbiol. 2016, 82(18):5642-5652.

6. Li Xu, Xiaohong Liu, Zhenhao Yin, Qian Liu, Lili Lu, Min Xiao *.Site-directed mutagenesis ofa-L-rhamnosidase fromAlternariasp. L1 to enhance synthesis yield of reverse hydrolysis based on rational design. Appl Microbiol Biotechnol. 2016, 100(24):10385-10394

7. Wei Gong，Li Xu，Guofeng Gu，Lili Lu，Min Xiao*. Efficient and regioselective synthesis of globotriose by a novela-galactosidase fromBacteroides fragilis. Appl Microbiol Biotechnol 2016,100(15): 6693–6702.

8. Shuquan Fan, Huaqin Zhang, Xiaodi Chen, Lili Lu, Li Xu and Min Xiao*. Cloning, characterization, and production of threea-L-fucosidases fromClostridium perfringensATCC 13124. J Basic Microbiol. 2015, 55(4):347-357.

9. Lili Lu, Qian Liu, Lan Jan, Zhenghao Yin, Li Xu, Min Xiao*. Enzymatic synthesis of rhamnose containing chemicals by reverse hydrolysis. PLoS One. 2015, 10(10):e0140531.

10. Lili Lu, Lijuan Xu, Yuchuan Guo, Dayu Zhang, Tingting Qi, Lan Jin, Guofeng Gu, Li Xu, Min Xiao*. Glycosylation of Phenolic Compounds by the Site-Mutated β-Galactosidase fromLactobacillus bulgaricusL3. PLoS ONE. 2015, 10(3): e0121445. doi:10.1371/journal.pone.0121445

11. Lili Zhang, Lili Lu, Shuquan Fan, Lan Jin, Guofeng Gu, Li Xu and Min Xiao*. One-step synthesis ofa-Gal epitope and globotriose derivatives by an engineereda-galactosidase. RSC Advances, 2015, 5(29), 22361 – 22364

12. Li Xu, Dongxue Wang, Lili Lu, Lan Jin, Jiawei Liu, Deyong Song, Zhongwu Guo, Min Xiao*. Purification, Cloning, Characterization, and N-Glycosylation Analysis of a Novelb-Fructosidase fromAspergillus oryzaeFS4 Synthesizing Levan- and Neolevan-Type Fructooligosaccharides. PLoS ONE. 2014, 9(12): e114793.

13. Lili Lu, Feng Fu, Renfei Zhao, Lan Jin, Chunjuan He, Li Xu, Min Xiao*. A recombinant levansucrase fromBacillus licheniformis8-37-0-1catalyzes versatile transfructosylation reactions. Process Biochem. 2014, 49(9): 1503-1510

14. Lili Lu, Jian Wu, Deyong Song, Han Zhao, Guofeng Gu, Yuchuan Guo, Jin Lan, Min Xiao*. Purification of fructooligosaccharides by immobilized yeast cells and identification of ethyl β-d-fructofuranoside as a novel glycoside formed during the process. Bioresour Technol, 2013, 132:365–369

15. Jinhua Zhang, Lili Lu, Lijie Yin, Shen Xie & Min Xiao*. Carotenogenesis gene cluster and phytoene desaturase catalyzing both three- and four-step desaturations fromRhodobacter azotoformans. FEMS Microbiol Lett, 2012, 333(2):138–145.

16. Qian Liu, Lili Lu, Min Xiao*. Cell Surface Engineering of α-L-rhamnosidase for Naringin Hydrolysis. Bioresour Technol, 2012, 123:144–149.

17. Lili Lu, Shuze Xu, Renfei Zhao, Dayu Zhang, Zhengyi Li, Yumei Li, Min Xiao*. Synthesis of galactooligosaccharides by CBD fusion beta-galactosidase immobilized on cellulose. Bioresour Technol, 2012, 116:327-333

18. Lili Lu, Shuze Xu, Lan Jin, Dayu Zhang, Yumei Li, Min Xiao*. Synthesis of galactosyl sucralose by beta-galactosidase fromLactobacillus bulgaricusL3. Food Chem, 2012, 134(1):269–275

Patents