Education

Research Experience

Research Interests

1.Bioremediation and utilization of environmental pollutants

Bioremediation and utilization of environmental pollutants include the application of nanotechnology inenvironmentalpollutantsand heavy metals bioremediation, the biodegradation of environmental pollutants in microbial fuel cells,and the extracellular electron transport mechanism ofelectroactivemicroorganisms.

2.Construction of biosensor based on nanomaterials

The immobilization of enzymes, microbial cells, and using nanomaterials as supports;theconstruction of biosensors based on the biocomposites constructed by nanomaterials and enzyme, microbial cells orantigen/antibody; the application of resulted biosensors in environmentalpollutantsmonitoring, food safety detection, and medical diagnosis.

Research Projects

1. 2021.01-2024.12, Nanowires-based extracellular electron transfer mechanismofSphingobiumyanoikuyae, National Natural Science Foundation of China

2.2020.01-2023.12,Study on the main sulfur-oxidizing bacterial groups and key pathways of sulfur oxidation in marine sediments (Study on sensitivity detection of sulfide by microbial sensor), Major Research plan of the National Natural Science Foundation of China

3. 2021.01-2024.12,Reconstruction of bioreactor monitoring system and optimization of industrial biological processes (subtopic),National Key Research and Development Program of China

4. 2019.01-2020.12,Study on sensitivity detection of sulfide by microbial sensor based on reductive graphene oxide/nano porous gold,Shandong Science & Technology Fund Planning Project.

5.2017.01-2019.12, Study on theoperational characteristic and mechanism of microfiltration bioreactor,The Fundamental Research Funds of Shandong University.

6.2016.01-2019.12,Microbial biosensor construction based on the expression of sulfide:quinone oxidoreductase for sulfide detection, National Natural Science Foundation of China.

7.2014.01-2016.12,Biosensor constructionbased on horseradish peroxidase-nanoporous gold and selective determination of phenols and aromatic amines in wastewater, Shandong Science & Technology Fund Planning Project.

8.2012.01-2015.12,Biodegradation of organic pollutants by microbial cells/nanoparticles biocomposite, National Natural Science Foundation of China.

9.2010.10-2013.10,Study on degradation of hexavalent chromium by nanoparticle/microbial cells biocomposites,Excellent Middle-Aged and YouthScientist Award Foundation of Shandong Province.

10.2008.01-2008.12,Structured kinetic study on the fermentation of gellan gum, National Natural Science Foundation of China.

11.2006.12-2010.12,Glutamine production byCorynebacterium glutamicumand optimization control of fermentation process, 863 Hi-Tech Research and Development Program of China.

Representative Publications

1.Xiao Sa, Shang Keshuai, Zhang Lei, Li Wenjuan,Wang Xia*.A rapid anti-Helicobacter pyloribiofilm drug screening biosensor based on AlpB outer membrane protein and colloidal gold/nanoporous gold framework.Biosens. Bioelectron.,2022, 215:114376.

2.Wang Huimin, Qi Xiaoyan, Zhang Lei, Zhang Xueli, Xu Ping,Wang Xia*.Efficient bioelectricity generation and carbazole biodegradation usingan electrochemically active bacteriumSphingobium yanoikuyaeXLDN2-5.Chemosphere, 2022, 307: 135986.

3. Shang Keshuai, Wang Shuangjue, Chen Siyu, Wang Xia*. Sensitivity detection of uric acid and creatinine in human urine based on nanoporous gold.Biosensors, 2022, 12: 588.

4.Siyu Chen, Keshuai Shang, Xinyu Gao,Xia Wang*.The development ofNAD⁺-dependent dehydrogenase screen-printed biosensor based on enzyme and nanoporous gold co-catalytic strategy.Biosens. Bioelectron., 2022,211:11437.

5.Huimin Wang, Xiaoyan Qi, Siyu Chen,Xia Wang*. The efficient treatment of breeding wastewater by an electroactive microbial community in microbial fuel cell.J.Environ. Chem. Eng., 2022, 10: 107187.

6.Sa Xiao,Keshuai Shang,Wenjuan Li, Xia Wang*. An efficient biosensor based on the synergistic catalysis ofHelicobacter pyloriurease b subunit and nanoplatinum for urease inhibitors screening and antagonistic mechanism analyzing.Sens. Actuators B Chem.,2022, 355:131284.

7.Yachao Zhang, Huimin Wang, KeshuaiShang, Xia Wang*, Ping Xu*.Reliable detection ofListeria monocytogenesby a portable paper-based multi-biocatalyst platform integrating three biomarkers: gene hly, acetoin,andlisteriolysin Oprotein.J. Electroanal. Chem., 2022, 905:115975.

8. Wang Shuangjue, Chen Siyu, Shang Keshuai, Gao Xinyu,Wang Xia*.Sensitive electrochemical detection of cholesterol using a portable paper sensor based on the synergistic effect of cholesterol oxidase and nanoporous gold.Int. J. Biol. Macromol., 2021, 189: 356-362.

9.XiaoSa, Wang Shuangjue,Wang Xia*, Xu Ping*. Nanoporousgold: areview andpotentials inbiotechnological andbiomedicalapplications.Nano Select,2021,2(8): 1437-1458.

10.Zhang Xueli,WangHuimin, XiaTian,Wang Xia*.Characterization of a new electrochemically active bacterium phylogenetically related toAlicyclobacillus hesperidumand its electrochemical performance in microbial fuel cell.Biosens. Bioelectron.,2021,175(3): 112865.

11. Bian Congcong, Wang Huimin, Zhang Xueli, Xiao Sa, Liu Zhuang,Wang Xia*. Sensitive detection of low-concentration sulfide based on the synergistic effect of rGO, np-Au, and recombinant microbial cell.Biosens. Bioelectron., 2020, 151(3): 111985.

12. Xia Tian, Zhang Xueli, Wang Huimin, Zhang Yachao, Gao Yan, Bian Congcong,Wang Xia*, Xu Ping. Power generation and microbial community analysis in microbial fuel cells: A promising system to treat organic acid fermentation wastewater.Bioresour. Technol., 2019, 284(7): 72-79.

13. Zhang Yachao, Gao Yan, Zhang Xueli, Wang Huiming, Xia Tian, Bian Congcong, Liang Shuang, Tang Xiaozhi,Wang Xia*.Electrochemical immunosensor for HBe antigen detection based on a signal amplification strategy: The co-catalysis of horseradish peroxidase and nanoporous gold.Sens. Actuators B Chem., 2019, 284(4): 296-304.

14. Liu Zhuang, Zhang Yachao, Bian Congcong, Xia Tian, Gao Yan, Zhang Xueli, Wang Huimin, Ma Hanyue, Hu Yuan,Wang Xia*.Highly sensitive microbial biosensor based on recombinantEscherichia colioverexpressing catechol 2,3-dioxygenase for reliable detection of catechol.Biosens. Bioelectron., 2019, 126(2), 51-58.

15. Ma Hanyue, Xia Tian, Bian Congcong, Sun Huihui, Liu Zhuang, Wu Chao,Wang Xia*, Xu Ping.Bacterial electroactivity and viability depends on the carbon nanotube-coated sponge anode used in a microbial fuel cell.Bioelectrochemistry, 2018, 122(8): 26-31.

16. Liu Zhuang, Ma Hanyue, Sun Huihui, Gao Rui, Liu Honglei,Wang Xia*, Xu Ping, Xun Luying. Nanoporous gold-based microbial biosensor for direct determination of sulfide.Biosens.Bioelectron., 2017, 98(12), 29-35.

17. Sun Huihui, Ma Hanyue, Liu Zhuang, Li Yufei, Xu Ping,Wang Xia*. Effect of Fe₃O₄nanoparticles onSphingobium yanoikuyaeXLDN2-5 cells in carbazole biodegradation.Nanotechnol.Environ. Eng.,2017, 2(12): 5.

18. Sun Huihui, Liu Zhuang, Wu Chao, Xu Ping,Wang Xia*. Amperometric inhibitive biosensor based on horseradish peroxidase-nanoporous gold for sulfide determination.Sci. Rep., 2016, 6(9), 30905.

19.Wu Chao, Liu Zhuang, Sun Huihui,Wang Xia*, Ping Xu. Selective determination of phenols and aromatic amines based onhorseradish peroxidase-nanoporous gold co-catalytic strategy.Biosens.Bioelectron., 2016, 79(3), 843-849.

20. Wu Chao, Sun Huihui, Li Yufei, Liu Xueying, Du Xiaoyu,WangXia*, Xu Ping.Biosensor based on glucose oxidase-nanoporous gold co-catalysis for glucose detection.Biosens.Bioelectron., 2015, 66(4), 350-355.

21. Wu Chao, Liu Xueying, Li Yufei, Du Xiaoyu,WangXia*, Xu Ping. Lipase-nanoporous gold biocomposite modified electrode for reliable detection of triglycerides.Biosens.Bioelectron., 2014, 53(3), 26-30.

22.Liu Xueying, Du Xiaoyu,WangXia*, Li Naiqiang, Xu Ping, Ding Yi. Improved microbial fuel cell performance by encapsulating microbial cells with a nickel-coated sponge.Biosens.Bioelectron.,2013, 41(3), 848-851.

23.Du Xiaoyu, Liu Xueying,Li Yufei,Wu Chao,WangXia*, Xu Ping. Efficient biocatalyst by encapsulating lipase into nanoporous gold.NanoscaleRes.Lett., 2013, 8(1), 180-185.

24. Li Yufei, Du Xiaoyu, Wu Chao, Liu Xueying,WangXia*, Xu Ping. An efficient magnetically modified microbial cell biocomposite for carbazole biodegradation.Nanoscale.Res.Lett., 2013, 8(1), 522-526.

25.Wang Xia, Liu Xueying, Yan Xiuling, Zhao Peng, Ding Yi*, Xu Ping*. Enzyme-Nanoporous gold biocomposite: excellent biocatalyst with improved biocatalytic performance and stability.PLoSOne, 2011, 6(9), e24207.

26.WangXia, Liu Xueying, Zhao Chuanming, Ding Yi*, Xu Ping*.Biodieselproduction in packed-bed reactors using lipase-nanoparticle biocomposite.Bioresour. Technol., 2011, 102(10), 6352-6355.

27.WangXia, Dou Peipei, Zhao Peng, Zhao Chuanming, Ding Yi*, Xu Ping*.Immobilization of lipases onto magnetic Fe₃O₄nanoparticles for application in biodiesel production.ChemSusChem, 2009, 2(10), 947-950.

28.WangXia,Zhao Chuanming, Zhao Peng, Dou Peipei, Ding Yi, Xu Ping*.Gellan gel beads containing magnetic nanoparticles: An effective biosorbent for the removal of heavy metals from aqueous system.Bioresour.Technol., 2009, 100(7), 2301-2304.

29.WangXia,Gai Zhonghui, Yu Bo,Feng Jinhui, Xu Changyong, Yuan Yong, Lin Zhixin, Xu Ping*.Degradation of carbazole by microbial cells immobilized in magnetic gellan gum gel beads.Appl.Environ.Microbiol., 2007, 73(20), 6421-6428.

30.WangXia, Yuan Yong, Wang Kainan, Zhang Dezhong, Yang Zhengting, Xu Ping*. Deproteinization of gellan gum produced bySphingomonas paucimobilisATCC 31461.J.Biotechnol., 2007, 128(2), 403-407.

31.WangXia,Xu Ping*, Yuan Yong, Liu Changlong, Zhang Dezhong, Yang Zhengting, Yang Chunyu, Ma Cuiqing.Modeling for gellan gum production bySphingomonas paucimobilisATCC 31461 in a simplified medium.Appl.Environ.Microbiol., 2006, 72(5), 3367-3374.

Honors and awards

1. Wang Xia,Shandong Excellent Master's Degree Thesis Guidance Award (2017)

2. Wang Xia,Shandong University Excellent Master's Degree Thesis Guidance Award (2017)

3. Wang Xia,National Excellent Doctoral Dissertation Nomination (2010)

4.WangXia,Excellent Doctoral Dissertations in Shandong Province (2009)

Patents

1.A method for detecting homocysteine in urine using an enzyme-modified electrode,Patent NoZL202110527576.3, China

2.A method for the screening ofHelicobacter pyloriurease b subunit inhibitors,Patent NoZL202110741469.0, China

3.A method for improved construction of biological electrodes using reduced graphene oxide nanomaterials,Patent NoZL202010037065.9, China

4. Thepreparation and application of a screen-printed electrode based on hemolysin OPatent NoZL202011126295.9, China

5.A dual-function sensor for detection ofListeria monocytogenesbased on detection ofhlygene and acetoin.Patent NoZL202010294110.9, China

6.A method for rapid detection of aromatic pollutants in wastewater by using nano-porous gold/horseradish peroxidase sensor. Patent NoZL201510888274.3, China

7.The application ofSphingomonas paucimobilisXLJ with high production in gellan gum fermentation.PatentNoZL200610068788.5, China

8. A Deproteinization method for fermentation liquid in gellan gum fermentation.Patent NoZL200610068787.0, China