Education

Research Experience

Research Interests

Current research interest isfocusing on metabolic engineering and synthetic biology of microorganisms. Specifically, these interest includes:

1) Pathway engineering of micro-organisms towards the efficient production of useful bulk chemicals and value added compounds;

2) Investigations of the molecular mechanisms of stress response during the metabolic engineering and fermentation process in these micro-organisms;

3) Developing the metabolic and biosynthetic methods or tools for the synthetic biology

Research Projects

2018-2022，NSFC project，construction of cell factory using non-homologous recombination

2017-2020，NSFC project，metabolism of UDP-GlcNAc in L. plantarum and its application in NeuAc prodcution

Representative Publications

Qian Wang, Jiasheng Xu, Zhijie Sun, Yaqi Luan, Ying Li, Junshu Wang, Quanfeng Liang, Qingsheng Qi:Engineering an in vivo EP-bifido pathway in Escherichia coli for high-yield acetyl-CoA generation with low CO 2 emission. Metabolic Engineering 08/2018; 51., DOI:10.1016/j.ymben.2018.08.003

Zhiyong Cui, Zhennan Jiang, Jinhong Zhang, Huihui Zheng, Xin Jiang, Kai Gong, Quanfeng Liang, Qian Wang, Qingsheng QI:Stable and efficient biosynthesis of 5-aminolevulinic acid using plasmid-free Escherichia coli. Journal of Agricultural and Food Chemistry 01/2019;, DOI:10.1021/acs.jafc.8b06496

Zhiyong Cui, Xin Jiang, Huihui Zheng, Qingsheng Qi, Jin Hou:Homology-independent genome integration enables rapid library construction for enzyme expression and pathway optimization in Yarrowia lipolytica: CUI et al.. Biotechnology and Bioengineering 11/2018;, DOI:10.1002/bit.26863

Zedao Liu, Jizhong Zhang, Jiao Jin, Zilong Geng, Qingsheng Qi, Quanfeng Liang:Programming Bacteria With Light—Sensors and Applications in Synthetic Biology. Frontiers in Microbiology 11/2018; 9:2692., DOI:10.3389/fmicb.2018.02692

Peng Yang, Jing Wang, Qingxiao Pang, Fengyu Zhang, Junshu Wang, Qian Wang, Qingsheng Qi:Pathway optimization and key enzyme evolution of N -acetylneuraminate biosynthesis using an in vivo aptazyme-based biosensor. Metabolic Engineering 08/2017; 43., DOI:10.1016/j.ymben.2017.08.001

Zhiyong Cui, Cuijuan Gao, Jiaojiao Li, Jin Hou, Carol Sze Ki Lin, Qingsheng Qi:Engineering of unconventional yeast Yarrowia lipolytica for efficient succinic acid production from glycerol at low pH. Metabolic Engineering 06/2017; 42., DOI:10.1016/j.ymben.2017.06.007

Xinyuan He, Yan Chen, Quanfeng Liang, Qingsheng QI:An autoinduced AND-gate controlling metabolic pathway dynamically in response to microbial communities and cell physiological state. ACS Synthetic Biology 12/2016; 6(3)., DOI:10.1021/acssynbio.6b00177

Tianyuan Su, Fapeng Liu, Pengfei Gu, Haiying Jin, Yizhao Chang, Qian Wang, Quanfeng Liang, Qingsheng Qi:A CRISPR-Cas9 Assisted Non-Homologous End-Joining Strategy for One-step Engineering of Bacterial Genome. Scientific Reports 11/2016; 6:37895., DOI:10.1038/srep37895

Cuijuan Gao, Xiaofeng Yang, Huaimin Wang, Cristina Perez Rivero, Chong Li, Zhiyong Cui, Qingsheng Qi, Carol Sze Ki Lin:Robust succinic acid production from crude glycerol using engineered Yarrowia lipolytica. Biotechnology for Biofuels 08/2016; 9(1):179., DOI:10.1186/s13068-016-0597-8

Dongfang Gao, Yaqi Luan, Quanfeng Liang, Qingsheng Qi:Exploring the N-terminal role of a heterologous protein in secreting out of Escherichia coli: A non-classical signal peptide in protein secretion. Biotechnology and Bioengineering 06/2016; 113(12)., DOI:10.1002/bit.26028

Peng Yang, Wenjing Liu, Xuelian Cheng, Jing Wang, Qian Wang, Qingsheng Qi:A new strategy for the production of 5-aminolevulinic acid in recombinant Corynebacterium glutamicum with high yield. Applied and Environmental Microbiology 02/2016; 82(9)., DOI:10.1128/AEM.00224-16

Peng Yang, Jing Wang, Qingsheng Qi:Prophage recombinases-mediated genome engineering in Lactobacillus plantarum. Microbial Cell Factories 10/2015; 14(1)., DOI:10.1186/s12934-015-0344-z

Yongzhen Xia, Wenqiao Chu, Qingsheng Qi, Luying Xun:New insights into the QuikChangeTM process guide the use of Phusion DNA polymerase for site-directed mutagenesis. Nucleic Acids Research 11/2014; 43(2)., DOI:10.1093/nar/gku1189

Quanfeng Liang, Qingsheng Qi:From a co-production design to an integrated single-cell biorefinery. Biotechnology Advances 11/2014; 32(7)., DOI:10.1016/j.biotechadv.2014.08.004

Qianqian Zhuang, Qian Wang, Quanfeng Liang, Qingsheng Qi:Synthesis of polyhydroxyalkanoates from glucose that contain medium-chain-length monomers via the reversed fatty acid β-oxidation cycle in Escherichia coli. Metabolic Engineering 05/2014; 24., DOI:10.1016/j.ymben.2014.05.004

Honors and awards

New century excellent talents of the ministry of education

Outstanding doctoral thesis guidance teachers of Shandong province

Patents

An engineered E.coli that can produce 5-aminolevulinic acid,PCT/CN2011/00I906