Education

Research Experience

Research Interests

1. Microbial Physiology and Biochemistry

2. Microbiome and Metabolic Interaction

3. Synthetic Biology and Biocatalysis

Research Projects

1. 2018.04-2021.06,Appliedbasicresearch on theMicrobial 2-hydroxycarboxylic acid catabolism,Shandong Provincial Funds for Distinguished Young Scientists

2. 2015.07-2020.06,Regulation mechanism of key enzymes in L-lactic acid metabolism ofPseudomonas aeruginosa,Young Scholars Program of Shandong University

3. 2015.01-2018.12,A new glutaric acid metabolic pathway inPseudomonas putida,National Natural Science Foundation of China

4. 2014.07-2016.12，Biotransformation of lysine to produce 5-aminovaleric acid，Chinese National Program for High Technology Research and Development

5. 2013.01-2016.12, Research on the catalytic mechanism and application ofanovel NAD-independent D-lactate dehydrogenase,National Natural Science Foundation of China

6. 2012.11-2015.12,Genomic scale system metabolic breeding of 2-butanolproducing bacteria,Chinese National Program for High Technology Research and Development

Representative Publications

1. Zhang Manman, Gao Chao*, Guo Xiaoting, Guo Shiting, Kang Zhaoqi, Xiao Dan, Yan Jinxin, Zhang Wen, Tao Fei, Dong Wenyue, Liu Pan, Yang Chen, Ma Cuiqing, Xu Ping*, Increased glutarate production by blocking the glutaryl-CoA dehydrogenation pathway and a catabolic pathway involving L-2-hydroxyglutarate. Nature Communications. 2018, 9,2114

2. Xiaoting Guo, Manman Zhang, Menghao Cao, Wen Zhang, Zhaoqi Kang, Ping Xu, Cuiqing Ma, and Chao Gao*.D-2-Hydroxyglutarate dehydrogenase plays a dual role in L-serine biosynthesis and D-malate utilization in the bacteriumPseudomonas stutzeri. J Biol Chem, 2018, 293,15513-15523.

3. Zhong Li, Jinxin Yan, Jinkai Sun, Ping Xu, Cuiqing Ma, and Chao Gao*.Production of value-added chemicals from glycerol using in vitro enzymatic cascades. Commun Chem, 2018, 1,71.

4. Zhang Wen, Zhang Manman, Gao Chao*, Zhang Yipeng, Ge Yongsheng, Guo Shiting, Guo Xiaoting, Zhou Zikang, Liu Qiuyuan, Zhang Yingxin, Ma Cuiqing, Tao Fei, Xu Ping*. Coupling between D-3-phosphoglycerate dehydrogenase and D-2-hydroxyglutarate dehydrogenase drives bacterial L-serine synthesis. Proc Natl Acad Sci U S A. 2017, 114, E7574-E7582.

5. Jiang Tianyi, Guo Xiaoting, Yan Jinxin, Zhang Yingxin, Wang Yujiao, Zhang Manman, Sheng Binbin, Ma Cuiqing, Xu Ping, Gao Chao*. A Bacterial Multidomain NAD-Independent d-Lactate Dehydrogenase Utilizes Flavin Adenine Dinucleotide and Fe-S Clusters as Cofactors and Quinone as an Electron Acceptor for d-Lactate Oxidization. J Bacteriol. 2017, 199, e00342-17.

6. Li Zhong, Zhang Manman, Jiang Tongtong, Sheng Binbin, Ma Cuiqing, Xu Ping, Gao Chao*. Enzymatic Cascades for Efficient Biotransformation of Racemic Lactate Derived from Corn Steep Water. ACS Sustainable Chem. Eng. 2017, 5, 3456-3464

7. Ge Yongsheng, Li Kun, Li Lixiang, Gao Chao*, Lijie Zhang, Ma Cuiqing*, Xu Ping, Contracted but effective: production of enantiopure 2,3-butanediol by thermophilic and GRASBacillus licheniformis. Green Chem, 2016, 18,4693-4703

8. Binbin Sheng, Jing Xu, Yongsheng Ge, Shuo Zhang, Danqi Wang, Chao Gao*, Cuiqing Ma, Ping Xu. Enzymatic Resolution by a d-Lactate Oxidase Catalyzed Reaction for (S)-2-Hydroxycarboxylic Acids. ChemCatChem 2016, 8, 2630-2633

9. Gao Chao, Li Zhong, Zhang Lijie, Wang Chao, Li Kun, Ma Cuiqing, Xu Ping, An artificial enzymatic reaction cascade for a cell-free bio-system based on glycerol, Green Chem, 2015, 17, 804-807

10. Gao Chao, Wang Yujiao, Zhang Yingxin, Lv Min, Dou Peipei, Xu Ping, Ma Cuiqing, NAD-Independent L-Lactate Dehydrogenase Required for L–Lactate Utilization inPseudomonas stutzeriA1501. J. Bacteriol, 2015, 197, 2239–2247

11. Sheng Binbin, Xu Jing, Zhang Yingxin, Jiang Tianyi, Deng Sisi, Kong Jian, Gao Chao*, Ma Cuiqing, Xu Ping, Utilization of D-Lactate as an Energy Source Supports the Growth ofGluconobacter oxydans, Appl Environ Microbiol. 2015, 81, 4098-4110

Honors and awards

1. 2019,Qilu Young Scholar of Shandong University

2. 2018,Shandong Provincial Funds for Distinguished Young Scientists

3. 2015,Young Scholars Program of Shandong University

Patents

1. A D-lactate oxidase and its application in the detection of D-lactic acid,Chinese invention patent, ZL201510135775.4

2. A process for producing chirally pure (2S,3S)-2,3-butanediol, Chinese invention patent, ZL 201110021066.5

3. A process for producing 2,3-butanediol and its specialBacillus licheniformis, Chinese invention patent, ZL 201110369152.5

4. One process for producing (2S,3S)-2,3-butanediol, and (3S)-acetoin from glucose, Chinese invention patent, ZL 201110247505.4

5. Directed evolution of L-lactate dehydrogenase and its application, Chinese invention patent, ZL 201210010514.6