Chao Gao

Release date:2019-06-21    Author:     Editor: liyuan    Click:








Shandong University

Fermentation Engineering



Shandong University


Research Experience





Shandong University



Shandong University

Associate Professor


Shandong University



Shanghaijiaotong University


Research Interests

1. Microbial Physiology and Biochemistry

2. Microbiome and Metabolic Interaction

3. Synthetic Biology and Biocatalysis

Research Projects

1. 2018.04-2021.06,Appliedbasicresearch on theMicrobial 2-hydroxycarboxylic acid catabolism,Shandong Provincial Funds for Distinguished Young Scientists

2. 2015.07-2020.06,Regulation mechanism of key enzymes in L-lactic acid metabolism ofPseudomonas aeruginosa,Young Scholars Program of Shandong University

3. 2015.01-2018.12,A new glutaric acid metabolic pathway inPseudomonas putida,National Natural Science Foundation of China

4. 2014.07-2016.12,Biotransformation of lysine to produce 5-aminovaleric acid,Chinese National Program for High Technology Research and Development

5. 2013.01-2016.12, Research on the catalytic mechanism and application ofanovel NAD-independent D-lactate dehydrogenase,National Natural Science Foundation of China

6. 2012.11-2015.12,Genomic scale system metabolic breeding of 2-butanolproducing bacteria,Chinese National Program for High Technology Research and Development

Representative Publications

1. Zhang Manman, Gao Chao*, Guo Xiaoting, Guo Shiting, Kang Zhaoqi, Xiao Dan, Yan Jinxin, Zhang Wen, Tao Fei, Dong Wenyue, Liu Pan, Yang Chen, Ma Cuiqing, Xu Ping*, Increased glutarate production by blocking the glutaryl-CoA dehydrogenation pathway and a catabolic pathway involving L-2-hydroxyglutarate. Nature Communications. 2018, 9,2114

2. Xiaoting Guo, Manman Zhang, Menghao Cao, Wen Zhang, Zhaoqi Kang, Ping Xu, Cuiqing Ma, and Chao Gao*.D-2-Hydroxyglutarate dehydrogenase plays a dual role in L-serine biosynthesis and D-malate utilization in the bacteriumPseudomonas stutzeri. J Biol Chem, 2018, 293,15513-15523.

3. Zhong Li, Jinxin Yan, Jinkai Sun, Ping Xu, Cuiqing Ma, and Chao Gao*.Production of value-added chemicals from glycerol using in vitro enzymatic cascades. Commun Chem, 2018, 1,71.

4. Zhang Wen, Zhang Manman, Gao Chao*, Zhang Yipeng, Ge Yongsheng, Guo Shiting, Guo Xiaoting, Zhou Zikang, Liu Qiuyuan, Zhang Yingxin, Ma Cuiqing, Tao Fei, Xu Ping*. Coupling between D-3-phosphoglycerate dehydrogenase and D-2-hydroxyglutarate dehydrogenase drives bacterial L-serine synthesis. Proc Natl Acad Sci U S A. 2017, 114, E7574-E7582.

5. Jiang Tianyi, Guo Xiaoting, Yan Jinxin, Zhang Yingxin, Wang Yujiao, Zhang Manman, Sheng Binbin, Ma Cuiqing, Xu Ping, Gao Chao*. A Bacterial Multidomain NAD-Independent d-Lactate Dehydrogenase Utilizes Flavin Adenine Dinucleotide and Fe-S Clusters as Cofactors and Quinone as an Electron Acceptor for d-Lactate Oxidization. J Bacteriol. 2017, 199, e00342-17.

6. Li Zhong, Zhang Manman, Jiang Tongtong, Sheng Binbin, Ma Cuiqing, Xu Ping, Gao Chao*. Enzymatic Cascades for Efficient Biotransformation of Racemic Lactate Derived from Corn Steep Water. ACS Sustainable Chem. Eng. 2017, 5, 3456-3464

7. Ge Yongsheng, Li Kun, Li Lixiang, Gao Chao*, Lijie Zhang, Ma Cuiqing*, Xu Ping, Contracted but effective: production of enantiopure 2,3-butanediol by thermophilic and GRASBacillus licheniformis. Green Chem, 2016, 18,4693-4703

8. Binbin Sheng, Jing Xu, Yongsheng Ge, Shuo Zhang, Danqi Wang, Chao Gao*, Cuiqing Ma, Ping Xu. Enzymatic Resolution by a d-Lactate Oxidase Catalyzed Reaction for (S)-2-Hydroxycarboxylic Acids. ChemCatChem 2016, 8, 2630-2633

9. Gao Chao, Li Zhong, Zhang Lijie, Wang Chao, Li Kun, Ma Cuiqing, Xu Ping, An artificial enzymatic reaction cascade for a cell-free bio-system based on glycerol, Green Chem, 2015, 17, 804-807

10. Gao Chao, Wang Yujiao, Zhang Yingxin, Lv Min, Dou Peipei, Xu Ping, Ma Cuiqing, NAD-Independent L-Lactate Dehydrogenase Required for L–Lactate Utilization inPseudomonas stutzeriA1501. J. Bacteriol, 2015, 197, 2239–2247

11. Sheng Binbin, Xu Jing, Zhang Yingxin, Jiang Tianyi, Deng Sisi, Kong Jian, Gao Chao*, Ma Cuiqing, Xu Ping, Utilization of D-Lactate as an Energy Source Supports the Growth ofGluconobacter oxydans, Appl Environ Microbiol. 2015, 81, 4098-4110

Honors and awards

1. 2019,Qilu Young Scholar of Shandong University

2. 2018,Shandong Provincial Funds for Distinguished Young Scientists

3. 2015,Young Scholars Program of Shandong University


1. A D-lactate oxidase and its application in the detection of D-lactic acid,Chinese invention patent, ZL201510135775.4

2. A process for producing chirally pure (2S,3S)-2,3-butanediol, Chinese invention patent, ZL 201110021066.5

3. A process for producing 2,3-butanediol and its specialBacillus licheniformis, Chinese invention patent, ZL 201110369152.5

4. One process for producing (2S,3S)-2,3-butanediol, and (3S)-acetoin from glucose, Chinese invention patent, ZL 201110247505.4

5. Directed evolution of L-lactate dehydrogenase and its application, Chinese invention patent, ZL 201210010514.6

Adress:State Key Laboratory of Microbial Technology, Shandong University, No. 72 Binhai Road, Qingdao 266237, P.R. China

Tel: (86)-0532-58631501  (86)-0532-58631597  Post Code: 266237

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