Shuning Wang    Professor    PhD Supervisor/    Master Supervisor

E-mail：shuningwang@sdu.edu.cn

Education

Degree	Date	Affiliation	Major
PhD	2000.09-2005.07	Shandong University	Microbiology
Bachelor	1996.09-2000.07	Shandong University	Microbiology

Research Experience

Date	Affiliation	Position
2005.07-present	State Key Lab of Microbial Technology, Shandong University	Lecturer (2005), Associate Professor (2007), Professor (2017)
2008.07-2010.08	Max Planck Institute for Terrestrial Microbiology	Postdoctoral Fellow
2011.04-2013.06	Max Planck Institute for Terrestrial Microbiology	Guest Professor

Research Interests

1. Biochemical and molecular mechanisms involved in the bioenergy production and carbon cycle by anaerobic bacteria and their application.

2. Microbial degradation of pollutants and its biochemical mechanisms.

3. Engineering the microbes to produce useful chemicals, biofuels, and enzymes.

Research Projects

1. 2022.01–2025.12, Mechanism of the promotion of acetic acid cycle on energy metabolism during syngas fermentation by Clostridium ljungdahlii, National Natural Science Foundation of China

2. 2020.01–2023.12, Function and mechanism of Rid6 subfamily protein in metabolite damage control in Agrobacterium tumefaciens, National Natural Science Foundation of China

3. 2015.01–2018.12, Unravelling the role of flavin-based electron bifurcation in energy metabolism of syngas fermentation by Clostridium autoethanogenum, National Natural Science Foundation of China

4. 2012.01–2015.12, The role of Redox-sensing repressor Rex in energy metabolism in Clostridium kluyveri, National Natural Science Foundation of China

5. 2010.01–2012.12, Physiology of nicotine degradation by Agrobacterium tumefaciens, National Natural Science Foundation of China

6. 2009.12– 2012.12, Cloning, expression and function analysis of key genes involved in nicotine degradation by Agrobacterium tumefaciens, the Excellent Middle-Aged and Youth Scientist Award Foundation of Shandong Province.

7. 2007.01–2009.12, Studying the nicotine degradation and transformation by Pseudomonas putida, National Natural Science Foundation of China

Representative Publications

1.Liang J.¹, Huang H., Wang Y., Li L., Yi J., Wang S*. (2022) A cytoplasmic NAD(P)H-dependent polysulfide reductase with thiosulfate reductase activity from the hyperthermophilic bacterium Thermotoga maritima. Microbiol. Spectr. 10(4): e0043622.

2.Wang, Y.¹, Zhang, M., Li, L., Yi, J., Liang, J., Wang, S.*, Xu P*. (2022) Biosynthesis of L-5-methyltetrahydrofolate by genetically engineered Escherichia coli. Microb. Biotechnol. 15(11): 2758-2772.

3.Shang J.¹, Wang X., Zhang M., Wang R., Zhang C., Huang H., Wang S*. (2021) Rid enhances the 6-hydroxypseudooxynicotine dehydrogenase reaction in nicotine degradation by Agrobacterium tumefaciens S33. Appl. Environ. Microbiol. 87(7): e02769-20.

4.Yi J.¹, Huang H., Liang J., Wang R., Liu Z., Li F., Wang S*. (2021) A heterodimeric reduced-ferredoxin-dependent methylenetetrahydrofolate reductase from syngas-fermenting Clostridium ljungdahlii. Microbiol. Spectr. 9: e00958-21.

5.Shang J.¹, Wang X., Zhang M., Li L., Wang R., Huang H., Wang S*. (2021) An NAD-specific 6-hydroxy-3-succinoyl-semialdehyde-pyridine dehydrogenase from nicotine-degrading Agrobacterium tumefaciens strain S33. Microbiol. Spectr. 9: e00924-21.

6.Huang H.¹, Shang J., Wang S*. (2020) Physiology of a hybrid pathway for nicotine catabolism in bacteria. Front. Microbiol. 11:598207.

7.Wang R.¹, Yi J., Shang J., Yu W., Li Z., Huang H., Xie H., Wang S*. (2019) 6-Hydroxypseudooxynicotine dehydrogenase delivers electrons to electron transfer flavoprotein during nicotine degradation by Agrobacterium tumefaciens S33. Appl. Environ. Microbiol. 85:e00454-19.

8.Liang J.¹, Huang H., Wang S*. (2019) Distribution, evolution, catalytic mechanism, and physiological functions of the flavin-based electron-bifurcating NADH-dependent reduced ferredoxin: NADP⁺ oxidoreductase. Front. Microbiol. 10:373.

9.Yu W. ¹, Wang R. ¹, Li H., Liang J., Wang Y., Huang H., Xie H., Wang S*. (2017) Green route to synthesis of valuable chemical 6-hydroxynicotine from nicotine in tobacco wastes using genetically engineered Agrobacterium tumefaciens S33. Biotechnol. Biofuels. 10:288.

10.Yu W.¹, Wang R., Huang H., Xie H., Wang S*. (2017) Periplasmic nicotine dehydrogenase NdhAB utilizes pseudoazurin as its physiological electron acceptor in Agrobacterium tumefaciens S33. Appl. Environ. Microbiol. 83(17):e01050-17.

11.Huang H^.1, Yu W., Wang R., Li H., Xie H., Wang S*. (2017) Genomic and transcriptomic analyses of Agrobacterium tumefaciens S33 reveal the molecular mechanism of a novel hybrid nicotine-degrading pathway. Sci. Rep. 7(1):4813.

12.Li H.¹, Xie K.¹, Yu W., Hu L., Huang H., Xie, H., Wang S*. (2016) Nicotine dehydrogenase complexed with 6-hydroxypseudooxynicotine oxidase involved in the hybrid nicotine-degrading pathway in Agrobacterium tumefaciens S33. Appl. Environ. Microbiol. 82(6): 1745–1755.

13.Yu W. ¹, Li H., Xie K., Huang H., Xie, H., Wang S*. (2016) Genome sequence of the nicotine-degrading Agrobacterium tumefaciens S33. J. Biotechnol. 228:1-2.

14.Hu L.¹, Huang H.¹, Yuan H., Tao F., Xie H., Wang S*. (2016) Rex in Clostridium kluyveri is a global redox-sensing transcriptional regulator. J. Biotechnol. 233:17-25.

15.Huang H.¹, Hu L., Yu W., Li H., Tao F., Xie H., Wang S*. (2016) Heterologous overproduction of 2[4Fe4S]- and [2Fe2S]-type clostridial ferredoxins and [2Fe2S]-type agrobacterial ferredoxin. Protein Expres. Purif. 121:1-8.

16.Demmer J. K.¹, Huang H.¹, Wang S., Demmer U., Thauer R. K.*, Ermler U*. (2015) Insights into flavin-based electron bifurcation via the NADH- dependent reduced ferredoxin: NADP oxidoreductase structure. J. Biol. Chem. 290(36): 21985-21995.

17.Li H.¹, Xie K.¹, Huang H., Wang S*. (2014) 6-Hydroxy-3-succinoylpyridine hydroxylase catalyzes a central step of nicotine degradation in Agrobacterium tumefaciens S33. PLoS ONE 9(7): e103324.

18.Mock J. ¹, Wang S., Huang H., Kahnt J., Thauer R.K*. (2014) Evidence for a hexaheteromeric methylenetetrahydrofolate reductase in Moorella thermoacetica. J. Bacteriol. 196:3303-3314

19.Wang, S.¹, Huang, H., Kahnt, J., Mueller, A., Köpke, M, Thauer, R. K*. (2013) NADP-specific electron-bifurcating [FeFe]-hydrogenase in a functional complex with formate dehydrogenase in Clostridium autoethanogenum grown on CO. J. Bacterial. 195:4373-4386

20.Wang S.¹, Huang, H., Kahnt, J., Thauer, R. K*. (2013) Clostridium acidurici electron-bifurcating formate dehydrogenase. Appl. Environ. Microbiol. 79: 6176-6179.

21.Wang S.¹, Huang H.¹, Kahnt J., Thauer R. K*. (2013) A reversible electron-bifurcating ferredoxin- and NAD-dependent [FeFe]-hydrogenase (HydABC) in Moorella thermoacetica. J. Bacteriol. 195:1267-1275. (¹ contributed equally)

22.Huang H.¹, Wang S.¹, Moll J., Thauer R. K*. (2012) Electron bifurcation involved in the energy metabolism of the acetogenic bacterium Moorella thermoacetica growing on glucose or H₂ plus CO₂. J. Bacteriol. 194:3689-3699. (¹ contributed equally)

23.Wang S.¹*, Huang H., Xie K., Xu P*. (2012) Identification of nicotine biotransformation intermediates by Agrobacterium tumefaciens strain S33 suggests a novel nicotine degradation pathway. Appl. Microbiol. Biotechnol. 95:1567-1578.

24.Wang S.¹, Huang H.¹, Moll J., Thauer R. K*. (2010) NADP⁺ reduction with reduced ferredoxin and NADP⁺ reduction with NADH are coupled via an electron-bifurcating enzyme complex in Clostridium kluyveri. J. Bacteriol. 192: 5115-5123. (¹ contributed equally)

25.Wang S. N.¹, Liu Z., Xu P*. (2009) Biodegradation of nicotine by a newly isolated Agrobacterium sp. strain S33. J. Appl. Microbiol. 107: 838-847.

26.Tang H. Z., Wang S. N., Ma L. Y., Meng X. Z., Deng Z. X., Zhang D. K., Ma C. Q., Xu P.* (2008) A novel gene encoding 6-hydroxy-3-succinoylpyridine hydroxylase in nicotine degradation by Pseudomonas putida strain S16. Appl. Environ. Microbiol. 74: 1567-1574.

27.Wang S. N.¹, Liu Z., Tang H. Z., Meng J., Xu P.* (2007) Characterization of environmentally friendly nicotine degradation by Pseudomonas putida biotype A strain S16. Microbiology-SGM 153: 1556-1565.

28.Wang S. N.¹, Xu P.*, Tang H. Z., Meng J., Liu X. L., Ma C. Q. (2005) ‘Green’ route to 6-hydroxy-3-succinoyl-pyridine from (S)-nicotine of tobacco waste by whole cells of a Pseudomonas sp. Environ. Sci. Technol. 39: 6877-6880.

29.Wang S. N.¹, Xu P.*, Tang H. Z., Meng J., Liu X. L., Huang J., Chen H., Du Y., Blankespoor H. D. (2004) Biodegradation and detoxification of nicotine in tobacco solid waste by a Pseudomonas sp. Biotechnol. Lett. 26: 1493-1496.

30.Wang S. N.¹, Du Y., Chen H., Xu P.* (2004) Research development of nicotine catabolism in microorganisms. China Biotechnology. 24(7): 50-54 (in Chinese)

Honors and Awards

1.Shandong University Excellent Doctoral Thesis, Shandong University, 2006.

2.Shandong Province Excellent Doctoral Thesis, Shandong Provincial Education Department, 2006.

3.Max Planck Society Foreigner Scholarship, 2008-2010.

4.Shandong University Excellent Course Website, Course: Microbial Fermentation Physiology, 2015.

5.Second place in the teaching competition in 2015-2016, Shandong University.

6.Shandong University Excellent Young Teacher, 2016.

7.Excellent oral presentation (Third Place) in Young Talent Meeting, State Key Lab of Microbial Technology, 2016.

Patents   

1.A biological method for synthesis of L-5-methyltetrahydrofolate. China Patent, ZL202110672088.1.

2.A recombinant E. coli and its fermentation for producing [2Fe2S]-ferredoxin. China Patent, ZL201510829212.5.

3.An Agrobacterium tumefaciens strain capable of degrading nicotine and its application. China Patent, ZL 200710013069.8

4.A Pseudomonas putida strain capable of degrading nicotine and its application. China Patent, ZL 200710013068.3

5.A biological method for synthesis of 6-hydroxy-3-succinoylpyridine from nicotine. China Patent, ZL 200510025598.0.