Website:www.mbtechinst.qd.sdu.edu.cn/info/1034/1725.htm
Website:www.mbtechinst.qd.sdu.edu.cn/info/1034/1725.htm
RESEARCH TOPICS
Archaea, DNA repair, Cell division, Host-virus interaction
EDUCATION
Ph.D. Cellular and Molecular Biology, University of Birmingham, UK, 1996
M.Sc. Botany,Lanzhou University, China, 1989
B.Sc. Botany,Lanzhou University, China, 1986
WORK EXPERIENCE
2003.11-present: Sate Key Laboratory of Microbial Resources, Professor;
1999.4-2003.10,Advanced Industrial Science and Technology (AIST), Ministry of Trade and Industry, Tsukuba, Japan, Researcher;
1997.1-1999.3, Institute of Fruit Tree Sciences, Ministry of Agriculture Fishery and Forestry, Japan Science and Technology (JST) Research Fellow;
1989.7-1993.8, Lanzhou Institute of Animal Husbandry Sciences, Chinese Academy of Agricultural Sciences, Assistant researcher.
RESEARCH AREAS
1. DNA repair in Archaea;
2. Mechanism of cell division, extracellular vesicle formation, and membrane coated virus exit in Sulfolobus;
3. Hos-virus interaction of Sulfolous;
4. Extremophiles resources and extremozyme.
PROJECTS
The research of this laboratory has been supported by grants from the National Science Foundation of China, Ministry of Science of Technology of China, and the State Key Laboratory of Microbial Technology.
REPRESENTATIVE PUBLICATIONS
1.Zhai B, DuPrez K, Han, H, Yuan Z, Ahmad S, Xu C, Gu L, Ni J, Fan L, Shen (2018) The archaeal ATPase PINA interacts with the helicase Hjm via its carboxyl terminal KH domain remodeling and processing replication fork and Holliday junction. Nucleic Acids Research. 46(13):6627-6641..
2. Liu J, Gao R, Li C, Ni J, Yang Z, Zhang Q, Chen H, Shen Y. (2017) Functional assignment of multiple ESCRT-III homologs in cell division and budding in Sulfolobus islandicus. Mol Microbiol. 105(4), 540–553.
3.Zhai B, DuPrez K, Doukov TI, Li H, Huang M, Shang G, Ni J, Gu L, Shen Y, Fan L. (2017) Structure and Function of a Novel ATPase that Interacts with Holliday Junction Resolvase Hjc and Promotes Branch Migration. J Mol Biol. 7;429(7):1009-1029.
4. Huang Q, Zhong Q, Mayaka J, Ni J, Shen Y (2017) Autophosphorylation and Cross-phosphorylation of Protein Kinases from the Crenarchaeon Sulfolobus islandicus. Frontiers in Microbiology, 8: 2173
5.Huang Q, Li Y, Zeng C, Song T, Yan Z, Ni J, She Q, Shen Y (2015) Genetic analysis of the Holliday junction resolvases Hje and Hjc in Sulfolobus islandicus. Extremophiles. 19:505-14.
6. Zheng T, Huang H, Zhang C, Ni J, She Q, Shen Y (2012) Development of simvastatin selection marker for hyperthermophilic acidophile, Applied. Environ. Microbiol. 78:568-574.
7. Hong Y, Chu M, Li Y, Ni J, Sheng D, Hou G, She Q, Shen Y (2012) Dissection of the functional domains of an archaeal Holliday junction helicase, DNA Repair. 11:102-111.
8. Li Z, Lu S, Hou G, Ma X, Sheng D, Ni J & Shen Y (2008) Hjm/Hel308a DNA helicase fromSulfolobus tokodaiipromotes replication fork regression and interacts with Hjc endonuclease in vitro. J Bacteriol. 190: 3006–17.
9. Shen, Y., Tang X.-F., Yokoyama H., Matsui E., and Matsui I. (2004) A 21-aa peptide from the cysteine cluster II of the family D DNA polymerase from Pyrococcushorikoshii stimulates its nuclease activity which is Mre11-like and prefers manganese ion as the co-factor. Nucleic Acids Research. 32: 158-168.
10.Shen Y, Tang XF, Matsui I. (2003) Subunit interaction and regulation of activity through terminal domains of the family D DNA polymerase from Pyrococcus horikoshii. J Biol Chem. 278(23):21247-57.
