Education
Degree |
Date |
Affiliation |
Major |
PhD |
1996.09-2001.06 |
Shandong University |
Microbiology |
Master |
1988.09-1991.06 |
Shandong University |
Microbiology |
Bachelor |
1984.09-1988.06 |
Shandong University |
Microbiology |
Research Experience
Date |
Affiliation |
Position |
2005.09-present |
State Key Laboratory of Microbial Technology, Shandong University, P. R. China |
Professor |
2000.09-2005.08 |
State Key Laboratory of Microbial Technology, Shandong University, P. R. China |
Associate Professor |
1991.07-2000.08 |
Department of Microbiology, Shandong University, P. R. China |
Lecturer and Assistant Lecturer |
1992.09-1993.05 |
Department of Agricultural Chemistry and Soil Science, University of Sydney, N.S.W. 2006, Australia |
Visiting Scholar |
Research Interests
1.Microbiology and molecular biology of extremely acidophilicAcidithiobacillus spp.
2. Mechanism, regulation and genetic modification of the ferrous iron oxidation, sulfur oxidation, heavy metal resistanceandorganic substrates metabolism in extremely acidophilicAcidithiobacillus spp.
3.Application ofAcidithiobacillus spp. on biomining, bio-desulfurization, recycling of wastemetalresources and so on.
Research Projects
1.2019-2022, Studyon thebifurcated electron transfer model of ferrous iron oxidation inAcidithiobacillus ferrooxidans ATCC 23270,Natural Science Foundation of China (31872621).
2.2016-2019, Studyand applicationonon the copper homeostatic mechanism ofAcidithiobacillus ferrooxidans ATCC 23270,Natural Science Foundation of China (31570041).
3.2014-2017, Study on the ferrous iron- and sulfur-oxidizing metabolism in the obligately chemolithoautotrophicAcidithiobacillus ferrooxidans,Natural Science Foundation of China(31370084).
4. 2010-2014, Application of the technology on the biological remove of hydrogen sulfide from industrial gas,Key Scientific and Technological Project of Shandong Province, P. R. China (2010GSF10626).
5. 2010-2012, Basic study on glucose metabolism by the obligately chemolithoautotrophicAcidithiobacillus ferrooxidans,Natural Science Foundation of China (30970025).
6. 2010-2013,Study on the knockout, replacement and function in glucose metabolism of the phosphofructokinase gene intheextremely acidophilic Acidithiobacillus ferrooxidans,Natural Science Foundation of Shandong Province, P. R. China (ZR2010CM004).
Representative Publications
1. Huiyan Wang, Liangyan Fang, Qing Wen, Jianqun Lin, Xiangmei Liu*.Application of β-glucuronidase (GusA) as an effective reporter for extremely acidophilicAcidithiobacillus ferrooxidans Appl. Microbiol. Biotechnol., 2017,101:3283-3294.
2. Huiyan Wang1, Shuangshuang Liu1,Xiangmei Liu*, Xiuting Li, Qing Wen, Jianqun Lin. Identification and characterization of an ETHE1-like sulfur dioxygenase in extremely acidophilicAcidithiobacillusspp.Appl. Microbiol. Biotechnol., 2014,98:7511-7522.
3. Qing Wen,Xiangmei Liu*, Huiyan Wang, Jianqun Lin. A versatile and efficient markerless gene disruption system forAcidithiobacillus thiooxidans: application for characterizing a copper tolerance related multicopper oxidase gene.Environ Microbiol, 2014, 16(11):3499-3514.
4.Yangyang Yu,Xiangmei Liu*, Huiyan Wang, Xiuting Li, Jianqun Lin.Construction and characterization of thetetH overexpression and knockout strains ofAcidithiobacillus ferrooxidans. J. Bacteriol. 2014, 196(12):2255-2264.
5.Jianzhou Meng1, Huiyan Wang1,Xiangmei Liu*, Jianqun Lin, Xin Pang, Jianqiang Lin. Construction of small plasmid vectors for use in genetic improvement of the extremely acidophilicAcidithiobacillus caldus.Microbiol. Res., 2013, 168:469-476.
6. Huiyan Wang,Xiangmei Liu*, Shuangshuang Liu, Yangyang Yu, Jianqun Lin, Jianqiang Lin, Xin Pang, and Jian Zhao. Development of a markerless gene replacement system forAcidithiobacillus ferrooxidans and construction of apfkB mutant.Appl. Environ. Microbiol., 2012, 78:1826-1835.
7. Likai Hao,Xiangmei Liu*, Huiyan Wang, Jianqun Lin, Xin Pang, Jianqiang Lin.Detection and validation of a small broad-host-range plamid pBBR1MCS-2 for use in genetic manipulation of the extremely acidophilicAcidithiobacillus sp.J. Microbiol. Methods, 2012, 90:309-314.
Patents
1.Xiangmei Liu, Qing Wen, Jianqun Lin. A copper resistant engineering strain ofAcidithiobacillusthiooxidans and its application. Patent number:ZL201410271443.4,National Invention, P. R. China. Approval date2016-07-06.
2.Xiangmei Liu, Qing Wen, Huiyan Wang, Jianqun Lin. A method for screening the markerless gene knockoutmutant of Acidithiobacillusthiooxidans. Patent number:ZL 201310029904.2,National Invention, P. R. China. Approval date2015-01-28.
3.Xiangmei Liu, Huiyan Wang, Yangyang Yu, Shuangshuang Liu, Jianqun Lin, Jianqiang Lin, Xin Pang.The markerless gene knockout method in extremely acidophilicAcidithiobacillus ferrooxidans. Patent number:ZL 201110188474.X,National Invention, P. R. China. Approval date2013-01-09.
